Priority Programme: THYROID TRANS ACT
Translation of Thyroid Hormone Actions beyond Classical Concepts

Prof. Dr. Josef Köhrle

Analytical procedures for the determination of the thyroid hormone status in biological samples collected in the framework of the priority programme SPP1629

Thyroid hormones (TH) and their metabolites (3,5-T2, 3-T1AM) with unexpected metabolic and cytoprotective functions have recently been identified and have attracted high attention among researchers, clinicians and the interested public. This development requires innovative, sensitive and robust analytical procedures beyond classical immunological or chromatographic techniques for quantification of pM TH concentrations in biological samples. With our LC-MS/MS method, many possible TH, thyronamines (TAM) and thyroacetic acid (TAC) can be measured. T4 and T3 can be reliably detected and quantified in single human serum samples. However, with the most sensitive LC-MS/MS system available on the market, it is now possible to additionally quantify rT3 and 3,5-T2 in human and mouse serum. The overall goal of providing these measurements as Z-service project for the SPP 1629 members, is to define physiological serum values for novel TH metabolites (TH/TAM/TAC) in relation to TSH. Moreover, we will gain a better insight into the biosynthesis, metabolism, transport, signalling and intracellular action of TH/TAM/TAC by analysing animal experimental tissue or cell culture samples.

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Project Coordinator(s):

Prof. Dr. Josef Köhrle
Charité - Universitätsmedizin Berlin
Institut für Experimentelle Endokrinologie
Augustenburger Platz 1
13353 Berlin
Phone: +49 30 450 524 022
Fax: +49 30 450 524 922
josef.koehrle@charite.de